Layer-specific analysis of cortical fear engram in mice

Ivashkina, O.; Toropova, K.; Roshchina, M.; Anokhin, K.

Авторы: Ivashkina Olga, Toropova Ksenia, Roshchina Marina, Anokhin Konstantin
Сборник: 2022 Fourth International Conference Neurotechnologies and Neurointerfaces (CNN)
Год издания: 2022
Первая страница: 59
Последняя страница: 62
DOI: 10.1109/CNN56452.2022.9912529
Аннотация: Associative learning is a fundamental mechanism for experience-dependent cortical modification. Though synaptic aspects of this process have been thoroughly studied, less is known about how it is implemented at the level of cortical circuits. Predictive coding theories suggest that classical conditioning leads to formation of cortical representations that differentially affect supragranular (L2/3) and infragranular (L5/6) cortical layers. We utilized the auditory fear conditioning paradigm and layer-specific c-Fos neuroimaging of associative prelimbic and cingulate) and auditory cortical areas after memory retrieval in mice to elucidate this issue. Retrieval of associative memory about auditory conditioned stimulus (CS) resulted in the preferential activation of cingulate and infralimbic cortices in conditioned mice compared to unpaired, CS-only controls and home cage mice. This activation was specific in L2/3, L5 and L6 of cingulate cortex and L5 and L6 of prelimbic cortex. In auditory cortical areas in all mice, presented with auditory stimuli during retrieval (conditioned, unpaired and sound control groups) there was strong a c-fos activation. Layer-specific analysis of the primary auditory cortex showed that this activation was proportional across all layers. Finally, we used correlation analysis and showed the c-fos expression in prelimbic and primary auditory but not cingulate cortex was highly-correlated across layers. The present findings indicate that associative memory cortical engram is unequal across layers and preferentially includes infragranular neurons.
Добавил в систему: Ивашкина Ольга Игоревна

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