Physiological Role of D-amino Acids and Bioanalytical Potential of D-amino Acid Oxidases

Tishkov, V.I.; Shelomov, M.D.; Pometun, A.A.; Savin, S.S.; Atroshenko, D.L.

Авторы: Tishkov V.I., Shelomov M.D., Pometun A.A., Savin S.S., Atroshenko D.L.
Журнал: Moscow University Chemistry Bulletin
Том: 78
Номер: 1
Год издания: 2023
Издательство: Allerton Press Inc.
Местоположение издательства: United States
Первая страница: 1
Последняя страница: 9
DOI: 10.3103/S0027131423010066
Аннотация: D-amino acid oxidase (DAAO) plays an important role in the functioning of both prokaryotes and eukaryotes. DAAO is increasingly being used in practice, including for the determination of D-amino acids in complex samples, including human tissues and fluids. There are generally two types of DAAO in all organisms. The first type is an enzyme highly specific for D-aspartate and has its own name D-aspartate oxidase (DASPO). DAAO of the second type is characterized by a wide spectrum of substrate specificity, with preference for one or another D-amino acid varying from source to source. The activity of DAAO with a large number of substrates greatly complicates the selective determination of a particular D-amino acid. The problem is often solved by choosing an enzyme that, under the conditions of analysis, has low or no activity with other D-amino acids present in the sample. For the convenience of selecting a particular enzyme, we have collected and analyzed literature data on the catalytic parameters of known DAAOs with the most important D-amino acids. In addition, similar data are presented for novel recombinant DAAOs from the methylotrophic yeast Ogataea parapolymorpha DL-1. Analysis of the data shows that, with the D-amino acid series, the new OpaDASPO and OpaDAAO have the highest catalytic parameters.
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Physiological Role of D-amino Acids and Bioanalytical Potential of D-amino Acid Oxidasesстатья

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