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A Method for the Production of Recombinant VSVs with Confirmation of Biological Activityстатья
Статья опубликована в журнале из списка Web of Science и/или Scopus
- Авторы: Moroz V.D., Gasanov N.B., Egorov A.D., Malogolovkin A.S., Nagornykh M.O., Subcheva E.N., Kolosova E.S., Fizikova A.Yu, Ivanov R.A., Karabelsky A.V.
- Журнал: Acta Naturae (англоязычная версия)
- Том: 16
- Номер: 1
- Год издания: 2024
- Издательство: Park Media Ltd
- Местоположение издательства: Moscow, Russia (Federation)
- Первая страница: 59
- Последняя страница: 66
- Аннотация: The design of new effective cancer treatment methods is a promising and important research field in translational medicine. Oncolytic viruses an induce immunogenic cell death by activating the body’s immune system to recognize tumor cells. This work presents the results for optimizing the production of recombinant vesicular stomatitis viruses (rVSVs). To ensure the assembly of viral particles, we developed the HEK293TN-T7 cell line, which stably expresses DNA-dependent RNA polymerase 7 for viral genome transcription, and obtained helper plasmids encoding viral genes under the control of the CAG promoter. The oncolytic activity of the purified virus preparation was assessed in a murine model of B16F10Red melanoma cells expressing a red fluorescent protein. The presented method makes it possible to obtain purified viral preparations with a high titer and oncolytic activity. The amplification of viral particles in a HEK293 suspension culture allows for rapid scalability. Therefore, the developed approach can be used to obtain other recombinant VSV-based oncolytic viruses for tumor immunotherapy.
- Добавил в систему: Егоров Александр Дмитриевич